Karyotyping of Human Chromosomes Using M_FISH Images

Quantitative karyotyping of human chromosomes by dual beam flow cytometry.

Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in bivariate flow karyotypes and study the impact of thi...

Karyotyping human and mouse cells using probes from single-sorted chromosomes and open source software.

Multispectral karyotyping analyzes all chromosomes in a single cell by labeling them with chromosome-specific probes conjugated to unique combinations of fluorophores. Currently available multispectral karyotyping systems require the purchase of specialized equipment and reagents. However, conventional laser scanning confocal microscopes that are capable of separating multiple overlapping emiss...

A Comparative Study and Karyotyping of Chromosomes of Hymenolepis nanafraterna from Mice and Rats

Automated Karyotyping of Metaphase Cells with Touching Chromosomes

Since the birth of the automated karyotyping systems by the aid of computers, building a fully automated chromosome analysis system has been an ultimate goal. Along with many other challenges, automating chromosome classification and segmentation has been a major challenge especially due to overlapping and touching chromosomes. The earlier reported methods have limited success as they are sensi...

Spectral karyotyping of human, mouse, rat and ape chromosomes--applications for genetic diagnostics and research.

Spectral karyotyping (SKY) is a widely used methodology to identify genetic aberrations. Multicolor fluorescence in situ hybridization using chromosome painting probes in individual colors for all metaphase chromosomes at once is combined with a unique spectral measurement and analysis system to automatically classify normal and aberrant chromosomes. Based on countless studies and investigation...